http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105461811-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-32122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-70539 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105461811-B |
| titleOfInvention | A kind of method of O-shaped foot-and-mouth disease virus polypeptide and SLA-2 heavy chain, the crystallization of light chain β 2m renaturation |
| abstract | The invention belongs to field of biotechnology, and in particular to a kind of O-shaped foot-and-mouth disease virus polypeptide and Hebao pig SLA-2 heavy chain, light chain β 2m renaturation, the method for crystallization.The present invention is by taking Hebao pig as an example, construct SLA-2 born of the same parents' outside area prokaryotic expression carrier, it is transferred to host strain and obtains expression albumen and its inclusion body, so that SLA-2, Hu62 polypeptide is correctly combined folding in vitro with s β 2m by dilution refolding method, molecular sieve is recycled to isolate and purify recombinant protein matter.Present invention employs prokaryotic expression system, height expresses heavy chain extracellular regions and the light chain of the SLA I molecule of Hebao pig, has many advantages, such as quick, intuitive, and the monocrystalline of SLA-2-HB-Hu62-s β 2m result acquisition high quality, crystal resolution ratio reaches , the asymmetrical bimolecular structure of SLA-2-HB-Hu62-s β 2m presentation, disclosing it may be there are two types of conformation. |
| priorityDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.