http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105448698-B

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filingDate 2015-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105448698-B
titleOfInvention The preparation method of phosphatide membrane array is patterned on a kind of indium tin oxide-coated glass
abstract The invention discloses the preparation method that phosphatide membrane array is patterned on a kind of indium tin oxide-coated glass, its step are as follows:(1)ITO surfaces TODS self assembly:Cleaned ITO electro-conductive glass is immersed in fresh TODS toluene solutions, taken out after 4 ~ 12h of sealing and standing, be put into 5 ~ 10min of ultrasonic cleaning in toluene, then cleaned the toluene on ITO surfaces with ethanol, finally dried up, be sealed stand-by with nitrogen;(2)The preparation of the ITO substrate of patterning:Chromium diaphragm plate containing patterning grid is placed on the ito glass of TODS self-assembled films modification, through 17 ~ 25min of ultra violet lamp;(3)50 ~ 200 μ L phosphatide vesicle solution are dropped in the ITO electrode surface of patterning, stand 30 ~ 60min under conditions of being 20 ~ 60 DEG C in temperature, the immobilized artificial membrane patterned in ITO electrode can be formed.Phosphatide film system prepared by this method can be characterized using fluorescence microscope and electrochemical method simultaneously, available for the two-dimentional membrane electrophoresis of charged species in research film, realize the enrichment and separation of charged species in immobilized artificial membrane.
priorityDate 2015-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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