http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105445453-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-543 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 2015-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105445453-B |
| titleOfInvention | Visualized immunoassay method based on nanogold growth |
| abstract | The invention discloses a visualized immunoassay method based on nanogold growth, which comprises the following steps: (1) adding antigens that have different concentrations and are capable of combining with specificity of an elisa plate into the elisa plate that is coated with a target acquisition antibody; (2) adding an enzyme labeled detection antibody into the elisa plate; (3) adding ascorbic acid ultraphosphate ester into the elisa plate, so that the ascorbic acid ultraphosphate ester reacts with labeled enzyme to generate ascorbic acid; (4) adding 20muL of the obtained reaction liquid to 220muL of gold rod growth liquid and then adding 10muL of gold core liquid, and after reaction, using a microplate reader for measuring absorption spectrum, and using a digital camera for recording the liquid color; (5) processing data and drawing a work curve; and (6) detecting and simulating a sample to be detected according to the above experimental steps. The method has the advantages that the operation is simple, the applicability is wide, the reaction is fast, various color changes can be generated, and the method can realize visualization quantitative determination for a target object. |
| priorityDate | 2015-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.