http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105441564-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2015-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105441564-B |
| titleOfInvention | Primer and method for detecting use condition of variable adenylate site of OSTC gene mRNA |
| abstract | The invention discloses a primer and a method for detecting the use condition of a variable adenylate site of OSTC gene mRNA. The primers include a first pair of primers and a second pair of primers, the sequences of which are identical to the two pairs of sequences in the 3' untranslated region of the OSTC gene or the complementary sequences thereof. The method comprises the following steps: extracting total RNA of a sample, and performing reverse transcription to obtain cDNA; using cDNA as a template, and using a first pair of primers qPCR to amplify the Proximal fragment of the common region to obtain a CT value pro (ii) a Amplifying the Disal fragment of the Extended region by using the second pair of primers and taking the cDNA as a template to obtain the CT value dis (ii) a Calculating CT value dis And CT value pro The ratio of (a) to (b). The method for detecting the use condition of the variable adenylate site of the OSTC gene mRNA is convenient and rapid, and is suitable for further popularization in laboratories with crude conditions and developing countries. |
| priorityDate | 2015-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.