http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105441533-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2014-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105441533-B |
titleOfInvention | PIK3CA detection in gene mutation system and its kit |
abstract | The invention discloses a kind of PIK3CA detection in gene mutation system and its kits, including primer, probe and amplification to block primer, and primer includes general forward primer, detection reverse primer and Quality Control reverse primer;Detection reverse primer include for the mutational site PIK3CA gene E542K detection reverse primer, for the detection reverse primer in the mutational site PIK3CA gene E545K, for the detection reverse primer in the mutational site PIK3CA gene H1047R, for the detection reverse primer in the mutational site PIK3CA gene H1047L;5 ' ends of probe sequence are equipped with a kind of fluorescent marker, and 3 ' ends of the nucleotide sequence of the probe are equipped with another fluorescent marker;Amplification blocks 3 ' ends of the nucleotide sequence of primer to be equipped with phosphorylation modification.PIK3CA detection in gene mutation system of the invention and its kit, can the mutational site E542K, E545K, H1047R or H1047L to PIK3CA gene detect, it is high sensitivity, high specificity, efficient and convenient. |
priorityDate | 2014-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 61.