http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105441415-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01041 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2457 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2016-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105441415-B |
| titleOfInvention | A kind of preparation method and applications of Pullulan enzymatic mutant PulB-d99-D436H |
| abstract | The invention discloses a kind of preparation method and applications of Pullulan enzymatic mutant PulB d99 D436H, its preparation method is the 100 926 amino acid peptide fragments for intercepting Nagano bacillus (Bacillus naganoensis) Pullulanase, the aspartic acid Asp in its 436 site is sported into histidine simultaneously, transformation obtains a new Pullulan enzymatic mutant.Advantage of the present invention：The mutant enzyme has excellent characteristic relative to the enzyme before mutation, and the expression quantity of wherein enzyme is 3.8 times of parent enzyme, and optimum temperature improves 2.5 DEG C, and catalytic activity is 1.36 times of parent enzyme.The mutant gene is integrated into bacillus subtilis (Bacillus subtilis) chromosome in a manner of homologous recombination and builds recombined bacillus subtilis, can steadily fermenting and producing Pullulanase.The mutant enzyme can improve the hydrolysis efficiency of starch and pulullan. |
| priorityDate | 2016-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 91.