http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105424918-A

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filingDate 2015-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105424918-A
titleOfInvention Immunoaffinity gel detection column for detecting fumonisins and preparation method thereof
abstract The invention provides a preparation method of an immunoaffinity gel detection column capable of visually and rapidly detecting fumonisins in food. The method comprises the steps that agarose gel is used as a solid-phase carrier, a fumonisins antibody and the agarose gel which is activated through cyanogen bromide are coupled to prepare antibody gel used as a detection layer, an HRP antibody and the agarose gel which is activated through the cyanogen bromide are coupled to prepare HRP antibody gel used as a quality control layer, the product is placed into 1mL of solid phase extraction column, and the immunoaffinity gel detection column is prepared. The novel immunoaffinity gel detection product which detects the fumonisins in the food rapidly, qualitatively and semiquantitatively is developed, and the detection limit is 40 micrograms/L. The immunoaffinity gel detection column has the outstanding advantages that specificity is high, and sensitivity is good; sample pretreatment is simple; detection time is short and accuracy is high; operation is easy and convenient, and assisting of large instruments is not needed.
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107807034-A
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