http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105424670-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-643 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 |
| filingDate | 2016-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105424670-B |
| titleOfInvention | A method for detecting 10-7-10-5M low-concentration H2O2 in solution or cells |
| abstract | The invention discloses a method for detecting 10-7-10-5 M low-concentration H 2 O 2 in solution or cells , belonging to the field of analytical chemistry. The compound (E)‑2‑(2,4‑dihydroxyphenyl)vinyl‑8‑hydroxyquinoline was used as a fluorescent probe for detecting trace amounts of H 2 O 2 . In acetonitrile/H 2 O solution (v/v, 3/2, pH 11), the characteristic fluorescence intensity or absorbance of the probe changes with the concentration of H 2 O 2 , and other coexisting oxidizing or reducing substances do not interfere with the determination. The concentration linear ranges of fluorescence method and ultraviolet absorption method for detecting H 2 O 2 were 8.3×10 ‑7 ~4.3×10 ‑5 M and 8.5×10 ‑7 ~5.2×10 ‑5 M, respectively, and the detection limits were 2.3×10 ‑8M and 8.9× 10‑7M . Visually detect H 2 O 2 , and the color of the probe solution changes from orange-red to colorless under sunlight and ultraviolet light. H 2 O 2 quenches probe fluorescence in living cells, and intracellular H 2 O 2 is visually detected using fluorescence imaging. |
| priorityDate | 2016-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.