http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105418741-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43504 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 |
| filingDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-10-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105418741-B |
| titleOfInvention | A kind of separation and purification method of leech polypeptide |
| abstract | The invention discloses a method for separating and purifying leech polypeptides. First, leech extracts are added to a system imitating the absorption of human intestinal cells for cultivation, and then the absorbed substances are chromatographically separated. The inventors of the present invention have solved the problem of separation and purification of leech polypeptides from different angles. By first purifying the leech extract with Caco-2 cells, the digestion and absorption process of the traditional Chinese medicine leech in the body has been imitated. Absorption, a large number of polypeptides that cannot be absorbed by human intestinal cells are first separated, which simplifies the separation and purification process, and then can be separated and purified by anion exchange column chromatography and gel filtration chromatography to be absorbed and utilized by the human body of polypeptides. The polypeptide isolated and purified by the method of the invention has the effect of inhibiting the migration of vascular smooth muscle cells. |
| priorityDate | 2016-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.