| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e9ad3aaf0fc9d311905f26b60422ca4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21062
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23L33-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-48 |
| filingDate |
2015-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74dfc4b5af4236377df7bacc5391e58a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1383310d47989523d59953320fc8c746
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36bf9e03cbcecb5ad786b3f7a6ccb2b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e23c6e56e1184e9b5513cb07ecb995d |
| publicationDate |
2016-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-105400761-A |
| titleOfInvention |
A kind of low molecular weight plasmin and its preparation method and application |
| abstract |
The invention relates to the technical field of biomedicine, and particularly discloses a low-molecular-weight plasmin and its preparation method and application. In the present invention, technologies such as ammonium sulfate salting out and column chromatography are used to separate the protease in the Bacillus subtilis fermentation broth; protein electrophoresis shows that the separated protein is a group of components with a molecular weight lower than 20kDa; The activity experiment proves that the low molecular weight plasmin can directly degrade fibrin and produce thrombolytic activity. The addition of trypsin does not affect its activity; the low molecular weight component is identified by peptide fingerprinting and protein N-terminal analysis technology, indicating that it is a group with the sequence table SEQ? ID? NO: A polypeptide composed of the amino acid sequence shown in 1-5. The fibrinolytic enzyme with thrombolytic activity provided by the invention has small molecular weight, strong activity, and is not degraded by trypsin, and has broad development prospects in the fields of health food, thrombolytic drug development and the like. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107828685-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109706092-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106749565-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106749565-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109706092-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109295042-A |
| priorityDate |
2015-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |