http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105385770-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105385770-B |
titleOfInvention | The Dual-ring hairpin probe for detecting bleomycin mediates label-free strand displacement amplification method |
abstract | The invention discloses a kind of Dual-ring hairpin probes of detection bleomycin to mediate label-free strand displacement amplification method, and in the presence of bleomycin, Dual-ring hairpin probe is broken at recognition site, and discharges trigger sequence;Then, trigger sequence is combined with the ring portion of signal probe, and under the action of polymerase and nicking enzyme, and strand displacement amplification reaction occurs, finally generates tetra- serobila sequences of a large amount of G.At the same time, primer chain extension opens signal probe, and the tetra- serobila sequences of G that signal probe neck is sealed up for safekeeping are exposed.Finally, tetra- serobila sequences of G bind NMM molecules, generate fluorescence signal, are quantified to bleomycin by the fluorescence signal detected.The present invention by trigger sequence design by, in the neck of Dual-ring hairpin probe, reducing the background signal of detection architecture;Bleomycin is cut and is combined with strand displacement amplification reaction, realizes that the Sensitive Detection to bleomycin, detection are limited to 0.34nM.This method has easy to operate, label-free and specific good advantage. |
priorityDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.