http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105385767-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6888 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2015-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105385767-B |
titleOfInvention | Primer and its identification method used in a kind of identification Eriocheir sinensis difference family |
abstract | The invention discloses primer and its identification methods used in a kind of identification Eriocheir sinensis difference family, comprising the following steps: acquires the sample of Eriocheir sinensis to be identified;DNA is extracted from Eriocheir sinensis leg muscle;Using the DNA extracted as template, PCR amplification is carried out with 10 pairs of micro-satellite primers;Electrophoresis detection is carried out to PCR product, then carries out silver staining;Digital assay is carried out to silver staining result, with the genetic distance between Genetics Software measurement individual, UPGMA clustering figure is drawn according to genetic distance, the individual of different familys is distinguished according to cluster analysis result.The present invention can fast and accurately distinguish the family of Eriocheir sinensis, effectively prevent inbreeding, there is very big application value in Eriocheir sinensis family selective breeding and breeding. |
priorityDate | 2015-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.