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publicationDate 2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105368943-A
titleOfInvention Kit and method for identifying mycobacterium strains
abstract The invention relates to a kit and a method for identifying mycobacterium strains. The method comprises the following steps: (1) design and synthesis of a primer and a probe; (2) PCR reaction component; (3) PCR amplification; and (4) melting curve analysis. The kit and the method disclosed by the invention, on the basis of fluorescent PCR and melting curve analysis technical platform, can detect 12 mycobacterium strains which are common in the clinical field through the innovative application of the novel double-labeled and double-specific probe which has nucleic acid modification, so that the Tm value difference among the various strains is more than or equal to 4 DEG C within at least one detection channel; and the kit and the method is suitable for accurately and effectively identifying a composite strain that two or more strains simultaneously exist.
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