Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b36b00cb92aade7643bf265dc2316b4c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4b9c4dabf0a7bb68a87c77c0a0bb2a71 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2015-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_709d991d30e7feac1d5800c01e6f7bed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ce10ca6338fbe82bc650d793af87cdc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea2a4f3866a8b34cef6cc77d9daff423 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49357e116e8900c17304a69da784509e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a5b00d493b9f82c882066631e4a6cea |
publicationDate |
2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-105368943-A |
titleOfInvention |
Kit and method for identifying mycobacterium strains |
abstract |
The invention relates to a kit and a method for identifying mycobacterium strains. The method comprises the following steps: (1) design and synthesis of a primer and a probe; (2) PCR reaction component; (3) PCR amplification; and (4) melting curve analysis. The kit and the method disclosed by the invention, on the basis of fluorescent PCR and melting curve analysis technical platform, can detect 12 mycobacterium strains which are common in the clinical field through the innovative application of the novel double-labeled and double-specific probe which has nucleic acid modification, so that the Tm value difference among the various strains is more than or equal to 4 DEG C within at least one detection channel; and the kit and the method is suitable for accurately and effectively identifying a composite strain that two or more strains simultaneously exist. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110872614-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106119363-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106834520-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106119363-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106868171-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106834520-A |
priorityDate |
2015-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |