http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105368930-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2015-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105368930-B |
| titleOfInvention | The determination method that enzymes combinations are sequenced in genotyping technique is sequenced |
| abstract | The present invention provides a kind of determination methods that enzymes combinations are sequenced in sequencing genotyping technique, include the following steps：(1) restriction enzyme digestion sites prediction is carried out to target genome, counts the endonuclease bamhi number that different digestion modes obtain；(2) joint sequence and PCR amplification primer sequence at every kind of endonuclease bamhi both ends are designed according to the endonuclease bamhi for the various digestion modes predicted in step (1)；(3) sequencing library is constructed using GBS technology for different digestion modes respectively；(4) it is sequenced using the sequencing library that step (3) construct；(5) SNP marker site is obtained according to sequencing result；(6) the specific enzymes combinations for being directed to target genome are determined according to different enzymes combinations SNP marker site number obtained, endonuclease bamhi size. |
| priorityDate | 2015-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 16.