http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105368829-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2015-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105368829-B |
| titleOfInvention | Universal/virulent dual fluorescence quantitative RT-PCR detection reagent, detection kit and detection method for Peste des petits ruminants virus |
| abstract | The invention relates to a universal/virulent dual fluorescence quantitative RT-PCR detection reagent, a detection kit and a detection method for Peste des petits ruminants virus, belonging to the technical field of animal quarantine. The detection reagents and kits of the present invention contain universal primers and probes for PPR and virulent primers and probes for PPR. The universal primers and probes use the specific sequence of the coding region of the PPR virus N gene as the target region, and the virulent primers and probes use the specific sequence of the coding region of the PPR virus H gene as the target region. The primer length is about 20 bases, the GC content is 50%-60%, there is no secondary structure and repeatability within the primer, there is no complementary sequence between primers and within the primer, and the difference in melting temperature (Tm value) between primers is less than 5℃ . The length of the probe is about 25 bases, and the Tm value is about 5°C higher than the Tm value of the primer, so that the PPR virus can be distinguished from other viruses, and the pest virus and the virulent virus can be distinguished. |
| priorityDate | 2015-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.