http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105368815-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2015-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105368815-B |
| titleOfInvention | The extracting method of polysaccharide polyphenol Plant Genome |
| abstract | The invention belongs to technical field of molecular biology, disclose a kind of extracting method of polysaccharide polyphenol Plant Genome, and step is：Nucleic acid dissociating buffer and 2 mercaptoethanols are added after taking vegetable material to grind, in mixing postposition water-bath；Supernatant is abandoned after being centrifuged in centrifuge；1 × PBS solution is added, shakes mixing on beveller, supernatant is abandoned after centrifugation；3 × CTAB the lysates and mixing of preheating, water-bath cracking are added into precipitation；Add isometric phenol/chloroform/isoamyl alcohol mixed liquor, mixing, centrifugation；Supernatant is taken, isometric chloroform/isoamyl alcohol mixed liquor, mixing, centrifugation is added；Supernatant is taken, NaCl and ice isopropanol is added, mixing precipitates 1~3 hour；After taking out centrifugation, precipitation is washed with ethyl alcohol, after air-drying plus TE dissolves, that is, completes extraction.The present invention can effectively remove polysaccharide polyphenol, reduce sugar and influence of the aldehydes matter to nucleic acid extraction, improve the quality and concentration of DNA. |
| priorityDate | 2015-11-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.