http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105352922-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2016-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105352922-B |
| titleOfInvention | A kind of Y shape DNA is assembled the detection method and detection kit that amplification of signal is used for mercury ion |
| abstract | The invention discloses a kind of Y shape DNA to be assembled detection method and detection kit of the amplification of signal for mercury ion, kit includes nucleotide sequence, buffer system, exonucleaseⅲ and double-stranded DNA fluorescent dyestuff, nucleotide sequence is made up of nucleotide sequence A, B, C, D, and A, B, C can be self-assembly of Y types complex and have 3 general protrusion area d；One group of unpaired T T couple at least be present after sequence D and general protrusion area's d complementary pairings, and protrude protection sequence.The system only needs single-cross to complete to react using Y shape DNA as sensing detection platform, simple to operate, cheap.Without marking modification, without separating, washing process, fast and easy detection.With exonuclease amplified signal, detection sensitivity is high, and selectivity is good.Detection of the inventive method to mercury is limited to 5pM, and the range of linearity is big from 10pM~100nM, the range of linearity. |
| priorityDate | 2016-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.