http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105352778-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105352778-B |
| titleOfInvention | A kind of preparation processing method for marine protein group study sample |
| abstract | The invention discloses a kind of preparation processing methods for marine protein group study sample.It is homogenized after lysate and protease inhibitors is added in marine organisms, is centrifuged, is taken supernatant and sediment；Sediment is subjected to cryogenic pulverization, ultrahigh speed low-temperature centrifugation obtains extracting solution of protein；Extracting solution of protein is subjected to acetone precipitation twice again, to protein group Sample Purification on Single, then through restoring, being alkylated, comprehensive type, concentration and the higher proteomics sample of purity are obtained in being organized from marine organisms.Present invention process is reasonable, operation is reliable, preparation science, have a wide range of application, recycle protein group to greatest extent in the case where bringing little damage to protein, and special optimization is carried out to the shotgun protein group Liquid Chromatography-Tandem Mass Spectrometry measurement based on isotope labelling absolute quantitation, the correlative study to carrying out marine protein matter group has greater significance. |
| priorityDate | 2015-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.