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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8286
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-325
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-325
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filingDate 2015-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2018-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2018-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105349555-B
titleOfInvention A kind of artificial synthesized Bt anti insect genes FLIa and its preparation method and application
abstract A kind of artificial synthesized Bt anti insect genes FLIa and its preparation method and application, belongs to insect resistance protein and Biosafety Technique field.Bt anti insect genes FLIa nucleotide sequence such as SEQ ID NO:Shown in 1, the SEQ ID NO in the amino acid sequence of its albumen encoded such as sequence table:Shown in 2.Bt anti insect genes FLIa preparation method is:Anti insect gene Cry1Ab Domain I and Domain II is swapped with anti insect gene Cry1Ia Domain III and merged, obtains restructuring anti insect gene MCry1I;On the basis of anti insect gene MCry1I is recombinated, anti insect gene Cry1Ja1 one of carbon tip is connected at 3 ' ends, obtains recombinating anti insect gene FLMCry1Ia;Codon transformation is carried out to restructuring anti insect gene FLMCry1Ia, obtains Bt anti insect genes FLIa.Bt anti insect genes FLIa can be stable hereditary in plant is converted and expressed, and expression quantity is high, and gained genetically modified plants insect resistace is good.The crops such as genetic transformation corn, cotton, rice, the vegetables, it is set to possess corresponding anti-insect activity, so as to reduce the usage amount of agricultural chemicals, to reduce environmental pollution and production cost.
priorityDate 2015-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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