http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105349528-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2015-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105349528-B |
| titleOfInvention | Library constructing method for full-length genome methylation weight bisulfite sequencing |
| abstract | The present invention provides a kind of library constructing method for full-length genome methylation weight bisulfite sequencing, and it is suitable for the original samples amounts of middleweight not (5~200ng).The library constructing method includes obtaining the genomic DNA of fragmentation from the genomic DNA of 5~200ng；Weight bisulf iotate-treated is carried out to the genomic DNA of above-mentioned fragmentation, obtains single-stranded heavy bisulf iotate-treated product；Using above-mentioned single-stranded heavy bisulf iotate-treated product as template, first chain is synthesized using the oligo1 primer containing biotin labeling；Use the single stranded DNA in system after first chain synthesis of exonuclease digestion；First chain of biotin labeling is transferred using streptavidin magnesphere；Using first chain of above-mentioned biotin labeling as template, Article 2 chain is synthesized using oligo2 primer；And PCR amplification is carried out using the Article 2 chain as template, so that DNA library is used in building two generations sequencing. |
| priorityDate | 2015-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.