http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105349441-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-885 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 |
filingDate | 2015-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2019-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105349441-B |
titleOfInvention | High sporogenic Trichoderma T23-Ovel1 bacterial strain and its construction method |
abstract | The invention discloses a kind of high sporogenic Trichoderma T23-Ovell bacterial strain and its construction method, building includes the building and ATMT conversion of vell gene overexpression frame;Tryptophan strong promoter is merged before the vell gene encoder block of Trichoderma atroviride bacterium T23 using over-lap PCR, tryptophan terminator is merged after vell gene encoder block, obtains vell gene overexpression frame;Vell gene overexpression frame is inserted into Trichoderma atroviride T23 genomic DNA by ATMT, obtains transformant;Transformant is verified, is screened.The present invention obtains the improved strain of high sporogenic Trichoderma, this method building is overexpressed bacterial strain production spore amount and is apparently higher than wild strain mainly from molecular genetic level by being overexpressed the transcription factor of sporogenesis.And the bacterial strain is by squamous subculture, and high yield conidium character can stablize heredity, provides excellent strain resource to develop efficient Trichoderma biocontrol agent. |
priorityDate | 2015-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.