http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105348392-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P29-00 |
| filingDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-08-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105348392-B |
| titleOfInvention | A kind of Nav1.7 inhibitor and its remodeling method |
| abstract | The present invention provides a kind of Nav1.7 inhibitor and its remodeling method, which belongs to fusion protein, including two parts: using SEQ ID NO:2 as the protein mutant of template and the polypeptide that can block Nav1.7 electric current.It is described using SEQ ID NO:2 as the protein mutant of template be sequence SEQ ID NO:2 14-31 by amino acid insertion, delete and displacement is formed by mutant.The present invention also provides a kind of remodeling methods: the inhibition polypeptide of NaV1.7 electric current can be blocked to link together with above-mentioned by the protein mutant of template of SEQ ID NO:2 by having, after merging with the MBP label protein in expression vector, it can go out to block the fusion protein of Nav1.7 electric current in Escherichia coli soluble-expression intracellular.The beneficial effects of the present invention are: the fusion protein can be in Escherichia coli carry out soluble-expression intracellular, purified product can block the Nav1.7 electric current on dorsal root ganglion (DRG) cell membrane, effect is similar to original polypeptide, and binding time is longer, it is more difficult to be eluted, and shows significant analgesic effect in rat experiment in vivo. |
| priorityDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.