http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105348384-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2417 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-805 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-125 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2015-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105348384-B |
| titleOfInvention | Vitreoscilla hemoglobin mutant and its controlled expression in genetic engineering bacterium |
| abstract | The invention discloses the recombined bacillus subtilis of the Vitreoscilla hemoglobin mutated gene after one plant of controlled expression codon optimization and its applications, the bacterial strain is recombined bacillus subtilis (Bacillus subtilis/PBE-Pxyl-vgb), and deposit number is CGMCC No.10787.Using the Vitreoscilla hemoglobin mutated gene vgh after codon optimization, bacillus subtilis expression vector PBE-Pxyl-vgb is constructed；Expression vector PBE-Pxyl-vgb electricity is transferred in bacillus subtilis, recombined bacillus subtilis (PBE-Pxyl-vgb) is obtained.When producing mesophilicα-diastase using this bacterial strain, xylose is added or is not added according to the needs of real attenuation process, to start or close the expression of Vitreoscilla hemoglobin, realize the controlled expression of Vitreoscilla hemoglobin gene, the excessive consumption for expressing this albumen always to nutriment in fermentation liquid and oxygen is avoided, thus improves recombined bacillus subtilis to the utilization efficiency of oxygen.The results show that the fermentation level of the mesophilicα-diastase of recombined bacillus subtilis improves 303% than original strain. |
| priorityDate | 2015-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.