http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105334196-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_49790ed5b8ddc5fa27a1d47732c612df |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2015-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea5049640b7c6c311315084395ab4ae9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8436a4ec70f70ed00ce06b5a68b206e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff063fef2cb0d5ab80ce5d486d92bf8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df16a630d01ad0110f76fef7fc4303e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ea7c1756b39fd63bb632e8464737f82 |
| publicationDate | 2016-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105334196-A |
| titleOfInvention | Method for detection of P-gp mediated Rh123 transport in 3D type organ and application thereof |
| abstract | The invention relates to a method for detection of P-glycoprotein (P-gp) mediated rhodamine 123 (Rh123) transport in a 3D type organ and an application thereof in screening of P-gp inhibitors. The method comprises the steps: firstly, sorting small intestinal crypts of C57BL/6 mice, inoculating a petri dish containing a matrix gel, culturing in an Advanced DMEM/F12 culture medium, and forming the 3D type organ; and then carrying out qualitative and quantitative detection of P-gp mediated Rh123 transport and influence of the P-gp inhibitor verapamil on Rh123 transport by using the 3D type organ model. The method particularly comprises the steps of respectively co-incubating the 3D type organ with (1) the P-gp substrate Rh123 and with (2) the Rh123 and the verapamil, releasing the Rh123 in the 3D type organ by using an ultrasonic crushing method, and finally detecting the concentration of the Rh123 on a multifunctional microplate reader. The method has the advantages of being simple, rapid, and high in sensitivity, can be combined with the 3D type organ model for carrying out research of the P-gp mediated drug transport, and also can perform research of in-vitro screening of the P-gp inhibitors. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105950539-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107012116-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109136188-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114317413-A |
| priorityDate | 2015-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.