http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105331729-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2013-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105331729-B |
| titleOfInvention | The method of SNP site genotyping |
| abstract | The invention discloses a kind of methods of SNP site genotyping.Method includes the following steps: S1, carries out cloning and sequencing and multiple alignment to the DNA sequence dna of target area, determines SNP site to be measured；S2 designs PCR amplification primer for SNP site, and the end 3' of forward primer or reverse primer in PCR amplification primer corresponds to SNP site to be measured, and the nucleotide on the end 3' of correspondence SNP site to be measured is carried out LNA modification；S3 is expanded using the DNA that PCR amplification primer treats test sample sheet, and the SNP site genotype of sample to be tested is determined according to the presence or absence of purpose band in amplified production.It applies the technical scheme of the present invention, lock nucleic acid primer is used to carry out PCR amplification to determine the genotype of SNP site, the accuracy and reliability of testing result are not only increased, and greatly improves the flexibility of experiment, simplify complementary conditions and significant reduces experimental cost. |
| priorityDate | 2013-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 19.