http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105319116-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 |
| filingDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105319116-B |
| titleOfInvention | Separation of protein N-terminal peptides based on magnetic microspheres |
| abstract | The invention belongs to the field of biotechnology, and in particular relates to a protein N-terminal separation method based on magnetic microspheres. The steps of the method of the present invention include: synthesizing magnetic nanoparticles; amino-blocking the protein, and then adding trypsin enzyme to obtain an enzymatic hydrolysis solution of the amino-blocking protein; reacting with Trout's reagent and the enzymolysis liquid of the amino-blocking protein, Non-N-terminal peptides were derivatized with sulfhydryl groups; magnetic nanoparticles were added to separate and enrich non-N-terminal peptides containing sulfhydryl groups; finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used for analysis and identification. The method of the invention can remove non-N-terminal peptides conveniently and efficiently, and improve the identification effect of protein N-terminal peptides. |
| priorityDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 145.