http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105316381-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-64 |
| filingDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105316381-B |
| titleOfInvention | Nanogold-modified graphene for protein N-terminal separation |
| abstract | The invention belongs to the field of biotechnology, in particular to a method for separating the N-terminus of proteins by using nano-gold modified graphene. The steps of the method of the invention are as follows: firstly synthesizing nano-gold modified graphene (G@PDA@Au), then enzymatically hydrolyzing the amino-blocked protein, derivatizing the enzymatic hydrolysis solution with sulfhydryl groups, using G@PDA@Au to derivatize the derivatized The non-N-terminal peptide segment of the upper thiol group was removed, and finally the N-terminal of the protein was detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The method of the invention can conveniently and efficiently remove the non-N-terminal peptide segment and improve the identification effect of the protein N-terminal peptide segment. |
| priorityDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 153.