http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105296521-B
Outgoing Links
Predicate | Object |
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classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
filingDate | 2015-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2020-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105296521-B |
titleOfInvention | Recombinant plasmid for expressing soluble human papilloma virus 16 subtype L1 protein and expression method thereof |
abstract | The invention discloses a recombinant plasmid for expressing soluble human papillomavirus 16 subtype L1 protein and an expression method thereof, wherein the recombinant plasmid is constructed by inserting a human papillomavirus 16 subtype L1 gene and a SUMO label gene into an expression plasmid. The invention optimizes the HPV16L1 gene, so that the gene is more suitable for expressing the target protein in an escherichia coli host with high efficiency, and simultaneously, the invention firstly applies a Sumo label expression system to perform fusion expression in the escherichia coli, so that the solubility of the target protein is further increased, and the expressed target protein has higher activity. The method of the invention obviously improves the soluble expression efficiency of the target protein, and the product has uniform and stable properties, the expression quantity of the target protein in the supernatant of the fermentation broth can reach 70 mu g/ml, and the value is obviously higher than the yield of the target protein in other prokaryotic expression systems, thereby meeting the requirements of industrial production. |
priorityDate | 2015-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.