| abstract |
The invention discloses DNA tags, PCR primers and applications thereof, wherein a set of DNA tags is selected from SEQ? ID? Nucleotides represented by NO: 1-95 can be used to construct nucleic acid sequencing libraries to accurately differentiate nucleic acid sequencing libraries. Using the DNA tag and PCR primers of the present invention to construct tag PCR primers, and then using the tag PCR primers, according to the method of the present invention for determining whether there are mutations in the deafness genes of multiple DNA samples, the deafness genes of 95 kinds of DNA samples can be realized at one time. Mutation detection. |