http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105296462-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-18 |
filingDate | 2015-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105296462-B |
titleOfInvention | A method of separating fixed a variety of enzymes |
abstract | A method of separating fixed a variety of enzymes, belongs to immobilised enzymes preparation field.The present invention is divided into following steps: enzyme A embedding in situ being fixed among the three-dimensional network of surface of polymer substrates first;Later under the irradiation of visible light, the photoinduction suspend mode kind for being fixed with the three-dimensional network layer surface of enzyme A regenerates surface free radical, cause the secondary active graft crosslinking polymerization of monomer solution progress for being mixed with enzyme B, enzyme B embedding in situ is fixed among the three-dimensional network of the first layer surface, second layer cross-linked network is formed;It repeats the above steps, different enzymes can be embedded in respectively among more network layers.The activity of most of enzyme can be saved in immobilization process.Different enzymes is separately fixed among different network layers, can not only be inhibited to avoid interfering with each other, but also a complicated chemical reaction can be completed jointly.The mechanical strength of composite membrane can be improved in polymeric substrate, enhances the stability of enzyme membrane, is more advantageous to the recycling and reusing of enzyme membrane. |
priorityDate | 2015-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 137.