http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105296409-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2015-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105296409-B |
| titleOfInvention | One plant of engineering bacteria and its construction method and application for producing immobilization alkaline pectase nanosphere |
| abstract | The invention discloses engineering bacterias and its construction method that one plant is used for One-step production alkaline pectin enzyme immobilization nanosphere, belong to enzyme immobilization technology field.The present invention constructs one plant of recombinant escherichia coli strain E.coli BL21 λ (DE3) pABC-PGL by the method for genetic recombination, is preserved in the common Bio-Centers of China Committee for Culture Collection of Microorganisms, and culture presevation number is CGMCC NO.10911.The present invention is based on the methods of recombinant escherichia coli strain disclosed above production immobilization alkaline pectase nanosphere：Utilize the method for Gene Fusion, current industrial widely applied alkaline pectinase gene is fused on the N-terminal of pha synthesizing enzyme gene phaC by link peptide, and this recombination segment is converted into recombinant bacterium and carries out inducing expression, energy synthetic surface has the nanosphere complex of alkaline pectase in recombinant bacterium body, alkaline pectase has just been effectively incorporated into nanosphere surface in this way, immobilization alkaline pectase is formed, a step realizes the production of alkaline pectin enzyme immobilizatio. |
| priorityDate | 2015-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 126.