http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105274082-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y305-01011 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P37-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P37-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
| filingDate | 2015-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105274082-B |
| titleOfInvention | A kind of synthesis penicillin G acylase mutant and its application in preparing Amoxicillin |
| abstract | The present invention provides a kind of synthesis penicillin G acylase mutant and its applications in preparing Amoxicillin.Half design and rational of fixed point saturation mutation technology and the enzyme engineering renovation technique of orthogenesis is combined to be mutated the penicillin G acylase in Achromobacter xylosoxidans source by CAD; it is lower to obtain hydrolysis vigor; synthesis of dynamic is more preferable; synthesize that hydrolysis ratio (S/H) higher, acid resistance be stronger and the better penicillin G acylase mutant of stability; can be more effective, rapidly catalyze and synthesize Amoxicillin.The 4 immobilized enzyme hydrolysis vigor of mutant SPGA that the present invention obtains has dropped 8.7 times, synthesis of dynamic improves 5.6 times, S/H values improve 8 times, 60min keeps 79% vigor under the conditions of pH2.0, catalyzes and synthesizes Amoxicillin using 10 DEG C or 20 DEG C of solid process, the wherein 6 direct solids of APA and D HPM of substrate feed intake without dissolving, pH is reacted without control, 300 batches or more can be used continuously 99% or more in substrate conversion efficiency, have good operational stability. |
| priorityDate | 2015-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 96.