http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105259168-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
| filingDate | 2015-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105259168-B |
| titleOfInvention | A kind of assay method of alkaline phosphatase activities |
| abstract | The invention discloses a kind of method for determining alkaline phosphatase activities.Catalysis substrate of the methods described using the phosphoric acid of L ascorbic acid 2 as alkaline phosphatase, the substrate is hydrolyzed to L ascorbic acid under the catalysis of alkaline phosphatase, because cupric can be reduced into monovalence copper by L ascorbic acid, the cupric bathophenanthroline compound of glassy yellow is converted into blackish green monovalence copper red phenanthroline compound, sample activity change of Alkaline phosphatase is higher, it is more obvious to detect liquid color change, so as to realize sxemiquantitative colorimetric detection to alkaline phosphatase activities or carry out quantitative detection with ultraviolet-uisible spectrophotometer.By the method for the present invention, 3.05mUmL can be detected ‑1 Alkaline phosphatase, compared with conventional method, this method high sensitivity, strong antijamming capability, reproducible, simple to operate, detection time is short, cost is cheap, and a kind of easy, cheap, high-resolution, sensitive and stable performance colorimetric methods are provided for the detection of clinical sample activity change of Alkaline phosphatase. |
| priorityDate | 2015-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 87.