http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105259006-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105259006-B |
| titleOfInvention | 4H- [1]-application of chromene [4,3-b] the thiophene -2-carboxylic acid acyl hydrazine and its derivative in the pre- dyeing detection method of glycoprotein specificity fluorescent |
| abstract | The present invention relates to the pre- dyeing detection method detection technique of glycoprotein specificity fluorescent, the application of 2 formic hydrazide of specifically 4H [1] chromene [4,3 b] thiophene and its derivative in the pre- dyeing detection method of glycoprotein specificity fluorescent.Present invention also offers the method that application 2 formic hydrazide of 4H [1] chromene [4,3 b] thiophene carries out the detection of glycoprotein specificity fluorescent pre-dyed, including step：Periodic acid solution is added into protein example to be aoxidized；Add in ascorbic acid solution and excessive periodic acid solution；Add dyestuff reaction；Add sample-loading buffer；Electrophoresis；Observation.The invention of this pre-dyed has the advantages that high sensitivity, selectivity are good, easy to operate rapidly, favorable reproducibility, linear relationship are good, mass spectrum is good, safe to use, of low cost.In addition relative to glyprotein stain method after gel electrophoresis, pre- dyeing detection method is observable after electrophoresis, saves gel electrophoresis poststaining step, can preferably be suitable for the research of high throughput protein group. |
| priorityDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.