http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105255932-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P29-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2015-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105255932-B |
| titleOfInvention | A kind of conotoxin variant GMVIIA and its preparation method and application |
| abstract | The invention discloses the preparation methods of conotoxin variant GMVIIA for having analgesic activities a kind of, belong to genetic engineering field；It is described the preparation method is as follows: according to e. coli codon frequency of use optimize conotoxin variant GMVIIA gene order, prokaryotic expression carrier pET-32a carrier is connected into after chemical synthesis, then recombinant plasmid is imported into Escherichia coli Origami(DE3) bacterial strain, after IPTG is induced, the solution expression with high efficiency of recombinant protein Trx-GMVIIA is realized；Recombinant protein Trx-GMVIIA is isolated and purified using nickel ion affinity chromatograph method；GMVIIA is released using enterokinase cutting recombinant protein Trx-GMVIIA, further Trx label is removed with nickel ion affinity chromatograph, obtains conotoxin variant GMVIIA.It is confirmed using mouse acetic acid writhing test, conotoxin variant GMVIIA prepared by the present invention has significant analgesic activities, has potential application in analgesia field. |
| priorityDate | 2015-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.