http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105255835-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2015-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105255835-B |
| titleOfInvention | It is a kind of to express the cell membrane particles for melting memebrane protein and its preparation and application |
| abstract | The present invention relates to a kind of express to melt the cell membrane particles and its preparation and application of memebrane protein, and VSVG is expressed in cell membrane particles；Cell membrane particles wrapping biological macromolecular and organelle；Cell membrane particles do not have nucleus.Preparation method: first with expression vesicular stomatitis virus albumen plasmid pair Ad293 cell transient transfection；Trypsin digestion cell is used after transfecting 48h, then is centrifuged 3min；Gained cell suspension is placed in syringe, and syringe is installed in the syringe-driven filter for having disposed polycarbonate membrane, push needle tubing make cell suspension from filter on one side release to it is other while, obtain expression VSVG cell membrane particles.The cell membrane particles that memebrane protein is melted in expression are applied in transmitting large biological molecule and organelle.Inventive film grains are bigger, energy wrapping biological macromolecular and mitochondria, functionally transferrin matter and mitochondria.Additional chemical reagent is not added, effectively maintains VSVG activity, improves after membrane granule enters inner body and discharges mitochondria efficiency, to improve mitochondria transmission efficiency. |
| priorityDate | 2015-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.