http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105238873-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
| filingDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105238873-B |
| titleOfInvention | A kind of detection method of amplification interior label and bacterium multiplex PCR |
| abstract | The present invention relates to the detection methods of a kind of amplification interior label and bacterium multiplex PCR.The nucleotide sequence of the amplification interior label is as shown in SEQ ID NO.1.The detection method includes the following steps: that (1) extracts the genomic DNA of sample to be tested, and DNA solution is made；(2) using genomic DNA as template, the amplification interior label is added in PCR amplification system, carries out PCR amplification, PCR product is made；(3) according to the PCR product after agarose gel electrophoresis, the result for judging whether there is false positive exists.Amplification interior label of the present invention is that the multiple object bacterias of multiplex PCR detection share, and not different target bacterium is obtained using the fusion of different amplification interior labels in the prior art, because its overall length is short, is synthesized difficulty and is substantially reduced, and detection effect stability and accuracy are high. |
| priorityDate | 2015-11-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.