http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105238849-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2425e03c2ac444c7ef5d68bb10d611cf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-54 |
| filingDate | 2015-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19b73e0374a7f93fb8b0fca4f3ea6ace http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eeb0456a4a2e11012fb7af6282d898cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_616fb97f5d3345c321f7180cb83e43b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a53d72407910ab798dbd983dafe5be81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f44464a20c2e9c37e078d3bf3855a76f |
| publicationDate | 2016-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105238849-A |
| titleOfInvention | A method for measuring the activity of sucrose invertase in lettuce guard cells |
| abstract | The invention provides a method for measuring the sucrose conversion enzyme activity of lettuce guard cells, comprising a step of preparing lettuce guard cell protoplasts; the separation of lettuce guard cells is carried out by an enzyme dissociation method; The step of protoplast protein; the obtained lettuce guard cell protoplast is extracted by acetone precipitation method; a step of measuring the lettuce guard cell protoplast protein, and the determination is determined by Coomassie Brilliant Blue G-250 method; A step for measuring the activity of sucrose invertase; the obtained guard cells measure the content of glucose, the product of the enzymatic chemical reaction, by an enzyme-linked method, and calculate the activity of sucrose invertase according to the amount of glucose produced, the reaction time, and the protein content of the guard cells. The invention solves the technical problem of difficulty in measuring the sucrose invertase activity of lettuce guard cells in the prior art. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106248664-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108624547-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106248664-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108624547-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110954492-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106554935-A |
| priorityDate | 2015-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 111.