http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105223351-B
Outgoing Links
Predicate | Object |
---|---|
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2014-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105223351-B |
titleOfInvention | Method and kit for rapidly detecting human group A streptococci based on magnetic separation and quantum dot labeling |
abstract | The invention provides a method for detecting a human group A streptococci antigen based on magnetic separation and quantum dot labeling. The method includes the steps: (1) preparing anti-human group A streptococci immune nano magnetic beads; (2) preparing quantum dot labeled anti-human group A streptococci nano probe; and (3) after dissolving a to-be-tested sample in a PBST buffer solution, adding the anti-human group A streptococci immune nano magnetic beads to the dissolved solution, fully mixing, carrying out a reaction, then carrying out magnetic separation, washing with a PBST buffer solution, adding the quantum dot labeled anti-human group A streptococci nano probe to the obtained precipitate, carrying out a reaction, then carrying out magnetic separation, washing with a PBST buffer solution, and then detecting a fluorescent value by using a fluorescence microplate reader. The accurate, rapid and high-sensitivity method for detecting human group A streptococci has quite high practical value in clinical diagnosis, etiology identification and epidemiological investigation of human M1, M3, M5, M6 and M24 serotype group A streptococci. |
priorityDate | 2014-08-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.