| abstract |
The invention provides an immobilized transaminase, which is obtained by immobilizing the transaminase with a histidine tag derived from mycobacterium (Mycobacterium? vanbaalenii) PYR-1 on an enzyme immobilized carrier, and the enzyme immobilized carrier It is obtained by derivatizing epoxy resin with iminodiacetic acid (IDA) and cobalt chloride in sequence, or derivatizing ion chelating resin with cobalt chloride. The immobilized transaminase of the present invention has firm combination, less loss of enzyme activity, simple separation, many times of reuse, low cost of asymmetric transformation process, mild reaction conditions, environmental friendliness, simple operation, easy industrial scale-up, and good industrial application prospect. |