http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105181948-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 2015-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105181948-B |
| titleOfInvention | Method for assaying luteoloside and special enzyme-linked immunosorbent assay kit thereof |
| abstract | The invention provides a method for assaying luteoloside and a special enzyme-linked immunosorbent assay kit thereof. The enzyme-linked immunosorbent assay kit provided by the invention includes an anti-luteoloside monoclonal antibody, and the monoclonal antibody is excreted by a hybridoma cell line CGMCC No.10581 which is screened out by the indirect competitive enzyme-linked immunosorbent assay method. An experiment proves that the specificity of the anti-luteoloside monoclonal antibody provided by the invention is high; the cross-reactivity to apigenin is 1.27 percent; the cross-reactivity to luteolin is 1.02 percent; the cross-reactivity to puerarin, naringin, hyperoside, isoorientin, rutin or quercetin is less than 0.001 percent. The enzyme-linked immunosorbent assay kit which is prepared by utilizing the monoclonal antibody can be used for qualitatively or quantitatively assaying the luteoloside in a sample to be assayed, the linear assay range is between 9.07ng/mL and 258.07ng/mL, and the enzyme-linked immunosorbent assay kit has the advantages of rapidness and sensitivity. |
| priorityDate | 2015-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.