http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105177076-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 |
| filingDate | 2015-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105177076-B |
| titleOfInvention | A kind of immobilised enzymes conversion DL-2- amino-△2The method of thiazoline -4- carboxylic acid synthesis L-cysteine |
| abstract | The invention discloses a kind of methods of immobilised enzymes conversion DL-2- amino-△ 2- thiazoline -4- carboxylic acid synthesis L-cysteine.The present invention passed through using polyvinyl alcohol-sodium alginate complex carrier be crosslinked-racemase containing DL-ATC, the fusion protein (SEQ ID NO.1) of L-ATC hydrolase and nitrogen-carbamyl-L-cysteine hydrolase (SEQ ID NO.4) fixed and obtain co-immobilization enzyme system by embedded-cross-linked technique, DL-ATC generation L-cysteine can be converted.Encoding fusion protein, nitrogen-carbamyl-L-cysteine hydrolase gene sequence respectively as shown in SEQ ID NO.2,5, will be described gene constructed to obtaining high-purity target protein by expression and purity on pET-28a.Co-immobilization enzyme system transformation efficiency of the invention, stability are high, have important industrial application value in the synthesis of L-cysteine. |
| priorityDate | 2015-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.