http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105175534-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-8121 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-57 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-81 |
| filingDate | 2015-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105175534-B |
| titleOfInvention | Application and preparation method thereof of the silkworm silk cocoon antifungal protein enzyme inhibitor in antimycotic |
| abstract | The application and its separation method that the invention discloses silkworm silk cocoon antifungal protein enzyme inhibitors in antimycotic, separation method is as follows: by silk cocoon layering, shredding, then the Tris-HCl buffer of pH7.5,100mM is added, extracts 30min under the conditions of 37 DEG C, revolving speed 220rpm；Then in room temperature, revolving speed is that 15min is centrifuged under 10000rpm, after 0.22 μm of membrane filtration of supernatant, obtains silkworm silk cocoon antifungal protein enzyme inhibitor；The protease inhibitors of acquisition is able to suppress the activity of the fungal proteinase K of woods Bai Shi Candida albicans secretion, inhibits the spore germination of silkworm pathogenic fungus beauveria bassiana, and still keeps activity after being incubated for 10min in boiling water；Belong to the antifungal protein of natural, high activity, high stability, it is nontoxic；Meanwhile the silk oilfields that the present invention utilizes are abundant, provide new way for the diversification development and utilization of silk. |
| priorityDate | 2015-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.