http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105153294-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-62 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 |
| filingDate | 2015-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105153294-B |
| titleOfInvention | A kind of recombinant insulin and insulin analog precursor purification method |
| abstract | The invention relates to the field of insulin production methods, and discloses a method for purifying recombinant insulin and insulin analog precursors. The present invention solves the problems that the conventional chromatography fillers existing in the purification and preparation of recombinantly expressed insulin precursors and insulin analog precursors cannot tolerate high salt loading, large amount of organic reagents, high cost and low product purity. , the present invention adopts the following technical scheme: after the fermentation broth supernatant after centrifugation is adjusted to pH and centrifuged, the chromatographic column prepared by any kind of filler in Capto S, Capto MMC, Uni PMM S, Uni MSP is directly loaded Adsorption, separation and purification, and elution are carried out to finally obtain high-purity recombinant insulin and insulin analog precursors; compared with the existing purification method, the present invention is simple in operation, high in yield, short in time consumption, small in impact on the environment, and can be used in large quantities. Reduce the cost of production of existing insulin and insulin analog products at scale. |
| priorityDate | 2015-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 135.