http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105145351-B

Outgoing Links

Predicate Object
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 2015-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2017-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-105145351-B
titleOfInvention The method of candidum tissue culturing forming seedling through one step culture mass production
abstract The invention discloses the method for candidum tissue culturing forming seedling through one step culture mass production, comprise the following steps:(1) breeding of protocorm:The terminal bud or axillary bud for selecting excellent iron sheet dendrobium plant are explant, after explant sterilization on MS+6 BA0.1~3.0mg/L+KT0.2~1.0mg/L+IAA0.1~1.0mg/L+NAA0.2~2.0mg/L+ cycocel 0.01~0.5mg/L Solid agar cultures, healthy and strong bud is differentiated while induction protocorm;(2) forming seedling through one step culture mass production:Directly being transferred on the Solid agar culture of MS+6 BA0.2~1.0mg/L+IAA0.2~0.5mg/L+NAA0.1~0.5~2.0mg/L of 2.0mg/L+ cycocels with bud protocorm of obtaining of step (1) is bred, broken up, strong sprout and is taken root;(3) test tube transplantation of seedlings.The method for tissue culture of short, low cost dendrobium candidum efficient low-consume amount the invention provides a kind of breeding cycle;Industrialized production is especially suitable for, and reaches industrialization level.
priorityDate 2015-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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