http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105132524-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44 |
filingDate | 2015-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105132524-B |
titleOfInvention | The dual amplification reaction of the cycle and DNAzyme cycles of Exo III auxiliary is used for Hg2+Detection |
abstract | The invention discloses the dual amplification reactions of the cycle of Exo III auxiliary and DNAzyme cycles to be used for Hg 2+ Detection, works as Hg 2+ In the presence of, the parts T-rich in H-DNA and A-DNA form T-Hg 2+ - T metal base-pairs, H-DNA hybridize with A-DNA, cause strand replacement reaction, form Hg 2+ The duplex structure (being known as Hg-Complex) of the protection part and the parts DNAzyme containing pendency mediated.Under the action of Exo III, Hg 2+ It is released with A-DNA, hybridizes with another H-DNA, recycled, obtain a large amount of DNAzyme sequences.Obtained DNAzyme sequences can recycle cutting molecular beacon, generate the signal of amplification.The effective combination recycled by two kinds, this strategy can sensitively detect the Hg in aqueous solution 2+ , and realize highly selective.Meanwhile the method can be successfully used to Hg in sewage 2+ Detection, be environmental water sample in Hg 2+ Quantitative detection provide potential tool. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109946279-A |
priorityDate | 2015-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.