http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105132390-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01005 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
filingDate | 2015-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105132390-B |
titleOfInvention | A kind of method that dextransucrase is prepared using mixed fungus fermentation |
abstract | The invention discloses a kind of method that dextransucrase is prepared using mixed fungus fermentation, it is characterised in that:The seed liquor of leuconostoc mesenteroide is inoculated into the fermentation medium containing the sucrose that mass concentration is 2%~5% first and is fermented, and the sucrose solution that mass concentration is 15% is added during the fermentation, continue mixed fungus fermentation in the seed liquor of 0~36h access spine spore Penicillium notatums of leuconostoc mesenteroide fermentation, gained zymotic fluid obtains dextransucrase crude enzyme liquid after film classified filtering.The method of the present invention prepares dextransucrase relative to leuconostoc mesenteroide fermentation is used alone, and mixed fungus fermentation can solve the problems, such as that dextransucrase separation is difficult, and can obtain more high enzymatic activity with more inexpensive, less energy consumption;Prepare dextransucrase relative to using engineering bacteria, the enzyme stability that mixed fungus fermentation is obtained is higher and simple to operate, the growth conditions and enzyme to bacterium to prepare facility requirements not high. |
priorityDate | 2015-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.