http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105112445-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105112445-B |
| titleOfInvention | A kind of miR-205 gene knockout kits based on CRISPR-Cas9 gene Knockouts |
| abstract | The 205 gene knockout kits of miR based on CRISPR Cas9 gene Knockouts that the invention discloses a kind of.The present invention passes through the drone design software preferably CRISPR Cas9 target sequences of a certain number of miR 205, and synthesis sgRNA is single-stranded in vitro, Insert Fragment is obtained by processing, then sgRNA is linked into using T4 ligases in plasmid vector, by transfecting LNCap cell strains, continue medicine sieve, fluoroscopic examination, obtains 205 Knockout cells strains of miR.By extracting cell DNA, PCR amplification miR 205 is simultaneously purified, PCR product is denaturalized, the mode of re-annealing forms Heterologous Hybridization double-strand, the shear efficiency for determining CRISPR Cas9 systems to miR 205 is tested using T7E1 digestions, the 205 gene knockout CRISPR Cas9 target sequences of miR of verification optimization are obtained, and build the orientation knockout that can be used for 205 genes of miR using kit with this.The kit has the characteristics that gene knockout is efficient, speed is fast, simple economy, has a extensive future in cell, animal model structure and medicine clinical research application aspect. |
| priorityDate | 2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.