http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105112355-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2015-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-105112355-B |
| titleOfInvention | A kind of cultural method of melanocyte |
| abstract | The invention discloses a kind of cultural methods of melanocyte.This method, which is included in front of melanocyte Multiplying culture, first carries out preculture 2-4h, the pre-culture medium are as follows: 2-4:1 is mixed DMEM culture medium by volume with Ham's F-12 culture medium；Adenine 1.6-2.0 × 10 ‑4 M；Penicillin 80-100 unit/mL；Streptomysin 80-100 μ g/mL；Hydrocortisone 0.1-0.5 μ g/mL；Insulin 2-6 μ g/mL；Epidermal growth factor 5-12ng/mL；Cholera toxin 0.8-1.5 × 10 ‑10 M；Fetal calf serum 4-7%.Preculture in the above method, it is adherent to promote melanocyte, improves melanocyte proportion and cell activity.1% is accounted for from initial melanocyte after passage, highest has been increased to 90%, and melanocyte form and function and stabilization characteristics of genetics. |
| priorityDate | 2015-09-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 159.