http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105092551-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
filingDate | 2015-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-105092551-B |
titleOfInvention | Nitric oxide production method is detected based on the fluorescence noble-metal nanoclusters that bovine serum albumin is modified |
abstract | Nitric oxide production method is detected the present invention relates to the fluorescence noble-metal nanoclusters modified based on bovine serum albumin, including:(1) the bovine serum albumin aqueous solution is configured, precious metal salt solution is added, it is 11~12 to be adjusted with sodium hydroxide to pH, is reacted 8~12 hours under the conditions of lucifuge, to prepare the fluorescence noble-metal nanoclusters of bovine serum albumin modification;(2) NO saturated solutions are configured, the NO standard liquids of various concentrations are diluted to, the concentration of each nitric oxide solution is surveyed with Griess reagent;(3) the fluorescence noble-metal nanoclusters and the nitric oxide solution reaction of various concentrations modified certain density bovine serum albumin, determine its fluorescence intensity under different NO concentration, the linear relationship between NO concentration and fluorescence intensity is built, the fluorescence noble-metal nanoclusters NO fluorescence probes modified based on bovine serum albumin are obtained.Compared with prior art, the present invention is convenient and simple, economical, and the fluorescence probe selectivity of preparation is high, and sensitivity is good, has huge application value to NO efficient detection. |
priorityDate | 2015-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.